Nuclear DNA Amount and Genome Size in Angiosperms

Evaluating the role of genome downsizing and size thresholds from genome size distributions in angiosperms.

PREMISE OF THE STUDY Whole-genome duplications (WGDs) can rapidly increase genome size in angiosperms. Yet their mean genome size is not correlated with ploidy. We compared three hypotheses to explain the constancy of genome size means across ploidies. The genome downsizing hypothesis suggests that genome size will decrease by a given percentage after a WGD. The genome size threshold hypothesis...

Nuclear DNA amounts in angiosperms: targets, trends and tomorrow.

BACKGROUND AND AIMS The amount of DNA in an unreplicated gametic chromosome complement is known as the C-value and is a key biodiversity character of fundamental significance with many practical and predictive uses. Since 1976, Bennett and colleagues have assembled eight compilations of angiosperm C-values for reference purposes and subsequently these have been pooled into the Angiosperm DNA C-...

Nuclear DNA content and genome size of trout and human.

NUCLEAR DNA CONTENT AND GENOME SIZE OF TROUT AND HUMAN In their recent paper, Thomas et al. (1) discussed design, resolution, sensitivity, and reproducibility of the National Aeronautics and Space Administration/American Cancer Society flow cytometer. The results of their study demonstrated high stability and sensitivity of the instrument, which is suitable for detection of near-diploid tumor c...

Plant DNA flow cytometry and estimation of nuclear genome size.

BACKGROUND DNA flow cytometry describes the use of flow cytometry for estimation of DNA quantity in cell nuclei. The method involves preparation of aqueous suspensions of intact nuclei whose DNA is stained using a DNA fluorochrome. The nuclei are classified according to their relative fluorescence intensity or DNA content. Because the sample preparation and analysis is convenient and rapid, DNA...

Nuclear DNA Content and Genome Size of American Ginseng